Saturday, July 2, 2011

Working in the Lab

In this post I talk about blood so for those of you who get grossed out by the sight of blood I have included the pictures at the end so you don't actually have to look at it.

In case anyone is confused about why I am in Berlin, let me explain. I am here on an internship with Humboldt University and I will be working with a PhD student on how different chromosomes affect fat content in the body. However, it is not currently ready so I have been working with another group of students on how pro-biotics (live microorganisms that may be beneficial) affect the immune system. They took pigs and gave each group a different type of pro-biotic and there was a control group. Then they collected tissue samples of things like the spleen, lymph nodes, etc. After the samples were collected is where my work came in. Some undergrad students in Berlin and I were working to isolate the lymphocytes, which will give us information on how the pro-biotics are affecting the immune system.

The first picture is me in the lab. The next two pictures are before and after the liquid was centrifuged to separate liquids of different densities. Once the densities were separated, we needed to remove the liquid in between the first and second layers of fluid (picture 4). After that we kept on centrifuging the liquid and re-suspending the liquid in a neutral solution until the lymphocytes were isolated.




2 comments:

Stephanie Pulham said...

MMM...bloody test tubes. Always a great post when it includes photos of pig blood.

Matt Sutter said...

I thought you were just going to be doing the statistical analysis stuff with the lab...not actual lab work. That's pretty cool!